FIGURE

Fig. 6

ID
ZDB-FIG-110701-5
Publication
Itou et al., 2011 - HMGB factors are required for posterior digit development through integrating signaling pathway activities
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

hmgb1and hmgb2 are required for enhancement of Wnt/β-catenin signaling in zebrafish embryos. Lateral views of 18-somite-stage embryos (A–E) and 36-hpf embryos (F–J) injected with control MO (A, F), hmgb1 MO (B, G), hmgb2 MO (C, H), both hmgb1 MO and hmgb2 MO (D, I) and hmgb1 MO, hmgb2 MO, Hmgb1 mRNA, and Hmgb2 mRNA (E, J). A–E: Top-GFP expression is visualized by in situ hybridization. Wnt/β-catenin signaling in forebrain (arrows), midbrain (asterisks), and tail bud (arrowhead) is visualized in control morphants (A), but downregulated in hmgb-downregulated embryos (B–D). In mRNA co-injected double morphants, Top-GFP expression is partially restored (E). Normal body extension (F) is impaired in hmgb1-morphants (G). The phenotype is less severe in hmgb2-morphants (H), but becomes more severe in double morphants (I). This phenotype was partially restored by co-injection of Hmgb1 mRNA and Hmgb2 mRNA (J).

Expression Data
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.