PUBLICATION

Regulation of slow and fast muscle myofibrillogenesis by Wnt/beta-catenin and myostatin signaling

Authors

Tee, J.M., van Rooijen, C., Boonen, R., and Zivkovic, D.

ID

ZDB-PUB-090616-54

Date

2009

Source

PLoS One 4(6): e5880 (Journal)

Registered Authors

van Rooijen, Carina

Keywords

Embryos, Muscle fibers, Muscle differentiation, Somites, Fast-twitch muscle fibers, Slow-twitch muscle fibers, Wnt signaling cascade, Zebrafish

MeSH Terms

Gene Expression Regulation, Developmental*
Cell Proliferation
Epistasis, Genetic
Cyclin-Dependent Kinase Inhibitor p21/metabolism
beta Catenin/metabolism*
Gene Expression Regulation*
Signal Transduction
Wnt Proteins/metabolism*
Hypertrophy
Myostatin/metabolism*
Muscles/embryology*
Zebrafish/embryology*
Muscle Fibers, Fast-Twitch/pathology*
Muscle Fibers, Slow-Twitch/pathology*
Animals

(all 15)

PubMed

19517013 Full text @ PLoS One

Abstract

Deviation from proper muscle development or homeostasis results in various myopathic conditions. Employing genetic as well as chemical intervention, we provide evidence that a tight regulation of Wnt/beta-catenin signaling is essential for muscle fiber growth and maintenance. In zebrafish embryos, gain-of-Wnt/beta-catenin function results in unscheduled muscle progenitor proliferation, leading to slow and fast muscle hypertrophy accompanied by fast muscle degeneration. The effects of Wnt/beta-catenin signaling on fast muscle hypertrophy were rescued by misexpression of Myostatin or p21(CIP/WAF), establishing an in vivo regulation of myofibrillogenesis by Wnt/beta-catenin signaling and Myostatin. Epistatic analyses suggest a possible genetic interaction between Wnt/beta-catenin and Myostatin in regulation of slow and fast twitch muscle myofibrillogenesis.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-lef1	standard conditions	Long-pec	somite decreased amount, abnormal	Fig. 7
apc^zf134/zf134; axin1^tm13/tm13	control	Long-pec	somite decreased amount, abnormal	Fig. 7
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Prim-25	myoblast proliferation increased occurrence, abnormal	Fig. 4
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Prim-25	myotome fast muscle cell disorganized, abnormal	Fig. 2
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Prim-25	myotome slow muscle cell hyperplastic, abnormal	Fig. 2
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Prim-25	myotome slow muscle cell hypertrophic, abnormal	Fig. 2
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Prim-25	skeletal muscle tissue development increased occurrence, abnormal	Fig. 4
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Prim-25	somite decreased amount, abnormal	Fig. 1
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Prim-25	whole organism decreased length, abnormal	Fig. 1
apc^zf134/zf134; axin1^tm13/tm13	standard conditions	Long-pec	myotome fast muscle cell disorganized, abnormal	Fig. 2

1 - 10 of 18

Show

Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
tm13		Point Mutation	axin1
w25Tg	Tg(top:GFP)	Transgenic Insertion
zf134		Point Mutation	apc

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
lef1	MO1-lef1	MRPHLNO
mstnb	MO1-mstnb	MRPHLNO

Fish

Fish
apc^zf134/zf134; axin1^tm13/tm13
apc^zf134/zf134; axin1^tm13/tm13 + MO1-lef1
apc^zf134/zf134; axin1^tm13/tm13; w25Tg
w25Tg
WT
WT + MO1-lef1

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Tee et al., 2009 ZDB-PUB-090616-54 PMID:19517013

Regulation of slow and fast muscle myofibrillogenesis by Wnt/beta-catenin and myostatin signaling

Tee et al., 2009

ZDB-PUB-090616-54
PMID:19517013