PUBLICATION
Brain-wide perception of the emotional valence of light is regulated by distinct hypothalamic neurons
- Authors
- Wagle, M., Zarei, M., Lovett-Barron, M., Poston, K.T., Xu, J., Ramey, V., Pollard, K.S., Prober, D.A., Schulkin, J., Deisseroth, K., Guo, S.
- ID
- ZDB-PUB-220430-2
- Date
- 2022
- Source
- Molecular psychiatry 27(9): 3777-3793 (Journal)
- Registered Authors
- Guo, Su, Prober, David, Wagle, Mahendra
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/metabolism
- Calcium
- Corticotropin-Releasing Hormone*/metabolism
- Hypothalamus/metabolism
- Mammals/metabolism
- Neurons/metabolism
- Paraventricular Hypothalamic Nucleus*/metabolism
- Perception
- Zebrafish/metabolism
- PubMed
- 35484242 Full text @ Mol. Psychiatry
Citation
Wagle, M., Zarei, M., Lovett-Barron, M., Poston, K.T., Xu, J., Ramey, V., Pollard, K.S., Prober, D.A., Schulkin, J., Deisseroth, K., Guo, S. (2022) Brain-wide perception of the emotional valence of light is regulated by distinct hypothalamic neurons. Molecular psychiatry. 27(9):3777-3793.
Abstract
Salient sensory stimuli are perceived by the brain, which guides both the timing and outcome of behaviors in a context-dependent manner. Light is such a stimulus, which is used in treating mood disorders often associated with a dysregulated hypothalamic-pituitary-adrenal stress axis. Relationships between the emotional valence of light and the hypothalamus, and how they interact to exert brain-wide impacts remain unclear. Employing larval zebrafish with analogous hypothalamic systems to mammals, we show in free-swimming animals that hypothalamic corticotropin releasing factor (CRFHy) neurons promote dark avoidance, and such role is not shared by other hypothalamic peptidergic neurons. Single-neuron projection analyses uncover processes extended by individual CRFHy neurons to multiple targets including sensorimotor and decision-making areas. In vivo calcium imaging uncovers a complex and heterogeneous response of individual CRFHy neurons to the light or dark stimulus, with a reduced overall sum of CRF neuronal activity in the presence of light. Brain-wide calcium imaging under alternating light/dark stimuli further identifies distinct and distributed photic response neuronal types. CRFHy neuronal ablation increases an overall representation of light in the brain and broadly enhances the functional connectivity associated with an exploratory brain state. These findings delineate brain-wide photic perception, uncover a previously unknown role of CRFHy neurons in regulating the perception and emotional valence of light, and suggest that light therapy may alleviate mood disorders through reducing an overall sum of CRF neuronal activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping