PUBLICATION

Chemokine receptor trafficking coordinates neutrophil clustering and dispersal at wounds in zebrafish

Authors
Coombs, C., Georgantzoglou, A., Walker, H.A., Patt, J., Merten, N., Poplimont, H., Busch-Nentwich, E.M., Williams, S., Kotsi, C., Kostenis, E., Sarris, M.
ID
ZDB-PUB-191116-7
Date
2019
Source
Nature communications   10: 5166 (Journal)
Registered Authors
Busch-Nentwich, Elisabeth, Sarris, Milka
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Cell Membrane/metabolism
  • Cell Movement
  • Down-Regulation
  • Endocytosis
  • Models, Biological
  • Mutagenesis/genetics
  • Mutation/genetics
  • Neutrophils/metabolism*
  • Protein Transport
  • Receptors, Interleukin-8A/chemistry
  • Receptors, Interleukin-8A/genetics
  • Receptors, Interleukin-8A/metabolism*
  • Receptors, Interleukin-8B/chemistry
  • Receptors, Interleukin-8B/genetics
  • Receptors, Interleukin-8B/metabolism*
  • Time Factors
  • Wounds and Injuries/pathology*
  • Zebrafish/metabolism*
PubMed
31727891 Full text @ Nat. Commun.
Abstract
Immune cells congregate at specific loci to fight infections during inflammatory responses, a process that must be transient and self-resolving. Cell dispersal promotes resolution, but it remains unclear how transition from clustering to dispersal is regulated. Here we show, using quantitative live imaging in zebrafish, that differential ligand-induced trafficking of chemokine receptors such as Cxcr1 and Cxcr2 orchestrates the state of neutrophil congregation at sites of tissue damage. Through receptor mutagenesis and biosensors, we show that Cxcr1 promotes clustering at wound sites, but is promptly desensitized and internalized, which prevents excess congregation. By contrast, Cxcr2 promotes bidirectional motility and is sustained at the plasma membrane. Persistent plasma membrane residence of Cxcr2 prolongs downstream signaling and is required for sustained exploratory motion conducive to dispersal. Thus, differential trafficking of two chemokine receptors allows coordination of antagonistic cell behaviors, promoting a self-resolving migratory response.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes