ZFIN ID: ZDB-PERS-090212-3
Sarris, Milka
Email: ms543@cam.ac.uk
Affiliation: Sarris Lab
Address: Department of Physiology, Development and Neuroscience University of Cambridge Downing Street, Anatomy Building CB2 3DY Cambridge
Country: United Kingdom

We are interested in how cells of the immune system navigate through tissue environments in order to find sites of infection or injury. This is essential for defence against invading microbes, while abnormal cell migration can also contribute to inflammatory diseases.
Immune cell behaviour has traditionally been interrogated in vitro and remarkably little is still known about how these cells search tissues and read guidance cues in situ. For example, it is unclear how different guidance cues propagate within living tissues, how they might establish functional concentration gradients and how gradients instruct cell behaviours. To investigate this at the cell biology level, we use a combination of live imaging in zebrafish, quantitative analyses of cell behaviour, genetic and optogenetic tools.

Georgantzoglou, A., Poplimont, H., Walker, H.A., Lämmermann, T., Sarris, M. (2022) A two-step search and run response to gradients shapes leukocyte navigation in vivo. The Journal of cell biology. 221(8)
Georgantzoglou, A., Coombs, C., Poplimont, H., Walker, H.A., Sarris, M. (2020) Live Imaging of Chemokine Receptors in Zebrafish Neutrophils During Wound Responses. Journal of visualized experiments : JoVE. (166)
Poplimont, H., Georgantzoglou, A., Boulch, M., Walker, H.A., Coombs, C., Papaleonidopoulou, F., Sarris, M. (2020) Neutrophil Swarming in Damaged Tissue Is Orchestrated by Connexins and Cooperative Calcium Alarm Signals. Current biology : CB. 30(14):2761-2776.e7
Coombs, C., Georgantzoglou, A., Walker, H.A., Patt, J., Merten, N., Poplimont, H., Busch-Nentwich, E.M., Williams, S., Kotsi, C., Kostenis, E., Sarris, M. (2019) Chemokine receptor trafficking coordinates neutrophil clustering and dispersal at wounds in zebrafish. Nature communications. 10:5166
Torraca, V., Gomes, M.C., Sarris, M., Mostowy, S. (2019) Meeting report: Zebrafish Infection and Immunity 2019. Lab animal. 48:284-287
Sarris, M., Olekhnovitch, R., Bousso, P. (2016) Manipulating leukocyte interactions in vivo through optogenetic chemokine release. Blood. 127:e35-41
Murayama, E., Sarris, M., Redd, M., Le Guyader, D., Vivier, C., Horsley, W., Trede, N., Herbomel, P. (2015) NACA deficiency reveals the crucial role of somite-derived stromal cells in haematopoietic niche formation. Nature communications. 6:8375
Deng, Q., Sarris, M., Bennin, D.A., Green, J.M., Herbomel, P., and Huttenlocher, A. (2013) Localized bacterial infection induces systemic activation of neutrophils through Cxcr2 signaling in zebrafish. Journal of Leukocyte Biology. 93(5):761-9
Sarris, M., Masson, J.B., Maurin, D., Van der Aa, L.M., Boudinot, P., Lortat-Jacob, H., and Herbomel, P. (2012) Inflammatory Chemokines Direct and Restrict Leukocyte Migration within Live Tissues as Glycan-Bound Gradients. Current biology : CB. 22(24):2375-2382

Munoz-Suano A*, Kallikourdis M.*, Sarris M.* , & Betz, A.G. Regulatory T cells suppress arthritis during pregnancy. Journal of Autoimmunity, 38, J103-8 (2012).
*These authors contributed equally

Sarris M. & Betz A.G. Live imaging of dendritic cell-Treg cell interactions.
Methods in Molecular Biology, 707, 83-101 (2011).

Sarris M. & Betz A.G. Shine a light: imaging the immune system. European Journal of Immunology 39, 1188-1202 (2009).

Sarris M., Andersen K.G., Randow F., Mayr, L. & Betz, A. G. Neuropilin-1 expression on regulatory T cells enhances their interactions with dendritic cells during antigen recognition. Immunity 28, 402-413 (2008).