PUBLICATION

Ectopically Expressed IL-34 Can Efficiently Induce Macrophage Migration to the Liver in Zebrafish

Authors
Jiang, Y., Chen, J., Yen, K., Xu, J.
ID
ZDB-PUB-190207-6
Date
2019
Source
Zebrafish   16(2): 165-170 (Journal)
Registered Authors
Chen, Jiahao, Jiang, Yunyun, Xu, Jin
Keywords
IL-34, macrophage, migration, zebrafish
MeSH Terms
  • Interleukins/genetics*
  • Interleukins/metabolism
  • Cell Movement/genetics*
  • Liver/physiology
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Macrophages/physiology*
  • Ectopic Gene Expression/physiology*
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Animals
(all 11)
PubMed
30724719 Full text @ Zebrafish
Abstract
Interleukin-34 (IL-34) is a known cytokine that plays an important role in the survival, proliferation, and differentiation of macrophages. In previous studies, IL-34 can induce macrophage migration through syndecan-1 or focal adhesion kinase and extracellular signal-related kinase 1 and 2 pathway. These studies mainly focused on in vitro experiments, but the effect of IL-34 on macrophage migration in vivo is less understood. In our study, we artificially induced macrophage, but not neutrophil, enrichment in the skin or liver by overexpressing IL-34 in epidermal cells or hepatocytes in zebrafish. Live imaging showed that the enrichment of macrophages in the liver is due to the direct attraction of macrophages by IL-34. Our results demonstrated that ectopically expressed IL-34 can induce macrophage migration to liver in vivo.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
hkz032tTg; szy101TgcontrolProtruding-mouth
hkz032tTg; szy101TgcontrolDay 4
hkz032tTg; szy101TgcontrolAdult
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
as3TgTransgenic Insertion
    nz117TgTransgenic Insertion
      szy101TgTransgenic Insertion
        hkz032tTgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Fish
          hkz032tTg; szy101Tg
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          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GFPEFGGFP
          1 - 2 of 2
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          Mapping
          No data available
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          Citation
          Jiang, Y., Chen, J., Yen, K., Xu, J. (2019) Ectopically Expressed IL-34 Can Efficiently Induce Macrophage Migration to the Liver in Zebrafish. Zebrafish. 16(2):165-170.