PUBLICATION
Ectopically Expressed IL-34 Can Efficiently Induce Macrophage Migration to the Liver in Zebrafish
- Authors
- Jiang, Y., Chen, J., Yen, K., Xu, J.
- ID
- ZDB-PUB-190207-6
- Date
- 2019
- Source
- Zebrafish 16(2): 165-170 (Journal)
- Registered Authors
- Chen, Jiahao, Jiang, Yunyun, Xu, Jin
- Keywords
- IL-34, macrophage, migration, zebrafish
- MeSH Terms
-
- Zebrafish/genetics
- Zebrafish/physiology*
- Interleukins/genetics*
- Interleukins/metabolism
- Macrophages/physiology*
- Cell Movement/genetics*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Ectopic Gene Expression/physiology*
- Animals
- Liver/physiology
- PubMed
- 30724719 Full text @ Zebrafish
Citation
Jiang, Y., Chen, J., Yen, K., Xu, J. (2019) Ectopically Expressed IL-34 Can Efficiently Induce Macrophage Migration to the Liver in Zebrafish. Zebrafish. 16(2):165-170.
Abstract
Interleukin-34 (IL-34) is a known cytokine that plays an important role in the survival, proliferation, and differentiation of macrophages. In previous studies, IL-34 can induce macrophage migration through syndecan-1 or focal adhesion kinase and extracellular signal-related kinase 1 and 2 pathway. These studies mainly focused on in vitro experiments, but the effect of IL-34 on macrophage migration in vivo is less understood. In our study, we artificially induced macrophage, but not neutrophil, enrichment in the skin or liver by overexpressing IL-34 in epidermal cells or hepatocytes in zebrafish. Live imaging showed that the enrichment of macrophages in the liver is due to the direct attraction of macrophages by IL-34. Our results demonstrated that ectopically expressed IL-34 can induce macrophage migration to liver in vivo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping