PUBLICATION
TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
- Authors
- Ladam, F., Stanney, W., Donaldson, I.J., Yildiz, O., Bobola, N., Sagerström, C.G.
- ID
- ZDB-PUB-180622-6
- Date
- 2018
- Source
- eLIFE 7: (Journal)
- Registered Authors
- Sagerström, Charles
- Keywords
- developmental biology, stem cells, zebrafish
- Datasets
- GEO:GSE102662
- MeSH Terms
-
- Blastula/embryology
- Blastula/metabolism
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/metabolism
- PubMed
- 29911973 Full text @ Elife
Abstract
TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-dependent gene regulatory network (GRN) required for anterior development and detect TALE occupancy associated with this GRN throughout embryogenesis. At blastula stages, we uncover a novel functional mode for TALE factors, where they occupy genomic DECA motifs with nearby NF-Y sites. We demonstrate that TALE and NF-Y form complexes and regulate chromatin state at genes of this GRN. At segmentation stages, GRN-associated TALE occupancy expands to include HEXA motifs near PBX:HOX sites. Hence, TALE factors control a key GRN, but utilize distinct DNA motifs and protein partners at different stages - a strategy that may also explain their oncogenic potential and may be employed by other broadly expressed TFs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping