ZFIN ID: ZDB-PUB-180622-6
TALE factors use two distinct functional modes to control an essential zebrafish gene expression program
Ladam, F., Stanney, W., Donaldson, I.J., Yildiz, O., Bobola, N., Sagerström, C.G.
Date: 2018
Source: eLIFE   7: (Journal)
Registered Authors: Sagerström, Charles
Keywords: developmental biology, stem cells, zebrafish
Microarrays: GEO:GSE102662
MeSH Terms:
  • Animals
  • Blastula/embryology
  • Blastula/metabolism
  • CCAAT-Binding Factor/genetics
  • CCAAT-Binding Factor/metabolism
  • Gene Expression Regulation, Developmental*
  • Gene Knockdown Techniques
  • Gene Regulatory Networks
  • Genes, Essential/genetics*
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism
  • Protein Binding
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 29911973 Full text @ Elife
TALE factors are broadly expressed embryonically and known to function in complexes with transcription factors (TFs) like Hox proteins at gastrula/segmentation stages, but it is unclear if such generally expressed factors act by the same mechanism throughout embryogenesis. We identify a TALE-dependent gene regulatory network (GRN) required for anterior development and detect TALE occupancy associated with this GRN throughout embryogenesis. At blastula stages, we uncover a novel functional mode for TALE factors, where they occupy genomic DECA motifs with nearby NF-Y sites. We demonstrate that TALE and NF-Y form complexes and regulate chromatin state at genes of this GRN. At segmentation stages, GRN-associated TALE occupancy expands to include HEXA motifs near PBX:HOX sites. Hence, TALE factors control a key GRN, but utilize distinct DNA motifs and protein partners at different stages - a strategy that may also explain their oncogenic potential and may be employed by other broadly expressed TFs.