PUBLICATION
Regulatory evolution of Tbx5 and the origin of paired appendages
- Authors
- Adachi, N., Robinson, M., Goolsbee, A., Shubin, N.H.
- ID
- ZDB-PUB-160810-7
- Date
- 2016
- Source
- Proceedings of the National Academy of Sciences of the United States of America 113(36): 10115-20 (Journal)
- Registered Authors
- Adachi, Noritaka, Shubin, Neil
- Keywords
- Tbx5 enhancer, development, evolution, paired fins
- MeSH Terms
-
- Animal Fins/anatomy & histology
- Animal Fins/growth & development
- Animal Fins/metabolism*
- Animals
- Animals, Genetically Modified
- Biological Evolution*
- Chromatin/chemistry
- Chromatin/metabolism
- DNA Footprinting
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental*
- Genes, Reporter
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Humans
- Jaw/anatomy & histology
- Lampreys/anatomy & histology
- Lampreys/classification
- Lampreys/genetics
- Lampreys/growth & development
- Phylogeny*
- T-Box Domain Proteins/genetics*
- T-Box Domain Proteins/metabolism
- Zebrafish/anatomy & histology
- Zebrafish/classification
- Zebrafish/genetics*
- Zebrafish/growth & development
- PubMed
- 27503876 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Adachi, N., Robinson, M., Goolsbee, A., Shubin, N.H. (2016) Regulatory evolution of Tbx5 and the origin of paired appendages. Proceedings of the National Academy of Sciences of the United States of America. 113(36):10115-20.
Abstract
The diversification of paired appendages has been a major factor in the evolutionary radiation of vertebrates. Despite its importance, an understanding of the origin of paired appendages has remained elusive. To address this problem, we focused on T-box transcription factor 5 (Tbx5), a gene indispensable for pectoral appendage initiation and development. Comparison of gene expression in jawless and jawed vertebrates reveals that the Tbx5 expression in jawed vertebrates is derived in having an expression domain that extends caudal to the heart and gills. Chromatin profiling, phylogenetic footprinting, and functional assays enabled the identification of a Tbx5 fin enhancer associated with this apomorphic pattern of expression. Comparative functional analysis of reporter constructs reveals that this enhancer activity is evolutionarily conserved among jawed vertebrates and is able to rescue the finless phenotype of tbx5a mutant zebrafish. Taking paleontological evidence of early vertebrates into account, our results suggest that the gain of apomorphic patterns of Tbx5 expression and regulation likely contributed to the morphological transition from a finless to finned condition at the base of the vertebrate lineage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping