PUBLICATION

Generation and Dynamics of an Endogenous, Self-Generated Signaling Gradient across a Migrating Tissue

Authors
Venkiteswaran, G., Lewellis, S.W., Wang, J., Reynolds, E., Nicholson, C., and Knaut, H.
ID
ZDB-PUB-131113-17
Date
2013
Source
Cell   155(3): 674-687 (Journal)
Registered Authors
Knaut, Holger, Lewellis, Stephen, Venkiteswaran, Gayatri, Wang, John
Keywords
none
MeSH Terms
  • Cell Movement
  • Lateral Line System/embryology*
  • Animals
  • Zebrafish Proteins/metabolism*
  • Morphogenesis
  • Models, Biological
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/analysis
  • Humans
  • Receptors, CXCR/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Chemokine CXCL12/metabolism
  • Signal Transduction
(all 14)
PubMed
24119842 Full text @ Cell
Abstract

In animals, many cells reach their destinations by migrating toward higher concentrations of an attractant. However, the nature, generation, and interpretation of attractant gradients are poorly understood. Using a GFP fusion and a signaling sensor, we analyzed the distribution of the attractant chemokine Sdf1 during migration of the zebrafish posterior lateral line primordium, a cohort of about 200 cells that migrates over a stripe of cells uniformly expressing sdf1. We find that a small fraction of the total Sdf1 pool is available to signal and induces a linear Sdf1-signaling gradient across the primordium. This signaling gradient is initiated at the rear of the primordium, equilibrates across the primordium within 200 min, and operates near steady state. The rear of the primordium generates this gradient through continuous sequestration of Sdf1 protein by the alternate Sdf1-receptor Cxcr7. Modeling shows that this is a physically plausible scenario.

Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sa16
    Point Mutation
    sk75TgTransgenic Insertion
      sk76TgTransgenic Insertion
        sk77TgTransgenic Insertion
          sk78TgTransgenic Insertion
            sk79TgTransgenic Insertion
              sk80TgTransgenic Insertion
                t26035
                  Point Mutation
                  t30516
                    Point Mutation
                    zf51TgTransgenic Insertion
                      1 - 10 of 11
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      ackr3aMO1-ackr3aMRPHLNO
                      ackr3aMO2-ackr3aMRPHLNO
                      cxcl12aMO2-cxcl12aMRPHLNO
                      1 - 3 of 3
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                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CeruleanEFGCerulean
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      mKate2EFGmKate2
                      1 - 4 of 4
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                      Mapping
                      No data available