PUBLICATION
Single nucleotide polymorphism (SNP) panels for rapid positional cloning in zebrafish
- Authors
- Clark, M.D., Guryev, V., Bruijn, E., Nijman, I.J., Tada, M., Wilson, C., Deloukas, P., Postlethwait, J.H., Cuppen, E., and Stemple, D.L.
- ID
- ZDB-PUB-110921-15
- Date
- 2011
- Source
- Methods in cell biology 104: 219-235 (Chapter)
- Registered Authors
- Clark, Matthew D., Cuppen, Edwin, Guryev, Victor, Postlethwait, John H., Stemple, Derek L., Tada, Masazumi, Wilson, Catherine
- Keywords
- zebrafish, strains, mapping, mutant, SNP, genotyping, MIPs, KASPar
- MeSH Terms
-
- Animals
- Base Sequence
- Chromosome Mapping/methods*
- Cloning, Molecular
- DNA/genetics
- DNA/isolation & purification
- DNA Probes/genetics
- Genetic Testing/methods
- Genome
- Genotype
- Larva
- Male
- Molecular Sequence Data
- Phylogeny
- Polymorphism, Single Nucleotide*
- Sequence Analysis, DNA
- Zebrafish/genetics*
- PubMed
- 21924166 Full text @ Meth. Cell. Biol.
Citation
Clark, M.D., Guryev, V., Bruijn, E., Nijman, I.J., Tada, M., Wilson, C., Deloukas, P., Postlethwait, J.H., Cuppen, E., and Stemple, D.L. (2011) Single nucleotide polymorphism (SNP) panels for rapid positional cloning in zebrafish. Methods in cell biology. 104:219-235.
Abstract
Despite considerable genetic and genomic resources the positional cloning of forward mutations remains a slow and manually intensive task, typically using gel based genotyping and sequential rounds of mapping. We have used the latest genetic resources and genotyping technologies to develop two commercially available SNP panels of thousands of markers that can be used to speed up positional cloning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping