PUBLICATION

Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut

Authors
Essner, J.J., Amack, J.D., Nyholm, M.K., Harris, E.B., and Yost, H.J.
ID
ZDB-PUB-050221-4
Date
2005
Source
Development (Cambridge, England)   132(6): 1247-1260 (Journal)
Registered Authors
Amack, Jeffrey, Essner, Jeffrey, Harris, Erin, Yost, H. Joseph
Keywords
Left-right patterning, Cilia, Kupffer’s vesicle, Dorsal forerunner cells, Left-right dynein, Organogenesis
MeSH Terms
  • Cilia/physiology
  • Gastrointestinal Tract/embryology*
  • Left-Right Determination Factors
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Brain/embryology*
  • Zebrafish/embryology*
  • Body Patterning/physiology
  • Heart/embryology*
  • Animals
(all 12)
PubMed
15716348 Full text @ Development
Abstract
Monocilia have been proposed to establish the left-right (LR) body axis in vertebrate embryos by creating a directional fluid flow that triggers asymmetric gene expression. In zebrafish, dorsal forerunner cells (DFCs) express a conserved ciliary dynein gene (left-right dynein-related1, lrdr1) and form a ciliated epithelium inside a fluid-filled organ called Kupffer's vesicle (KV). Here, videomicroscopy demonstrates that cilia inside KV are motile and create a directional fluid flow just prior to the onset of asymmetric gene expression in lateral cells. Laser ablation of DFCs and surgical disruption of KV provide direct evidence that ciliated KV cells are required during early somitogenesis for subsequent LR patterning in the brain, heart and gut. Antisense morpholinos against lrdr1 disrupt KV fluid flow and perturb LR development. Furthermore, lrdr1 morpholinos targeted to DFC/KV cells demonstrate that Lrdr1 functions in these ciliated cells to control LR patterning. This provides the first direct evidence, in any vertebrate, that impairing cilia function in derivatives of the dorsal organizer, and not in other cells that express ciliogenic genes, alters LR development. Finally, genetic analysis reveals novel roles for the T-box transcription factor no tail and the Nodal signaling pathway as upstream regulators of lrdr1 expression and KV morphogenesis. We propose that KV is a transient embryonic 'organ of asymmetry' that directs LR development by establishing a directional fluid flow. These results suggest that cilia are an essential component of a conserved mechanism that controls the transition from bilateral symmetry to LR asymmetry in vertebrates.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
dnah9noton1/n1 (AB)standard conditionsShieldISH
dnah9sox32ta56/ta56standard conditions75%-epibolyNot DetectedISH
dnah9tbx16b104/b104 (AB)standard conditions75%-epibolyISH
dnah9tbxtab195/b195standard conditions75%-epibolyNot DetectedISH
dnah9tdgf1m134/m134 (AB)standard conditions75%-epibolyNot DetectedISH
dnah9WTstandard conditions10-13 somitesISH
dnah9WTstandard conditions75%-epibolyISH
dnah9WTstandard conditions75%-epibolyISH
dnah9WTstandard conditions90%-epibolyISH
dnah9WTstandard conditions5-9 somitesISH
1 - 10 of 66
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    		Indel
    b195
      		Insertion
      m134
        		Point Mutation
        m768
          		Point Mutation
          n1
            		Small Deletion
            ta56
              		Point Mutation
              tf219
                		Point Mutation
                ty68b
                  		Point Mutation
                  1 - 8 of 8
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  dnah9MO1-dnah9MRPHLNO
                  dnah9MO2-dnah9MRPHLNO
                  lft1MO2-lft1MRPHLNO
                  1 - 3 of 3
                  Show
                  Fish
                  Fish
                  foxh1m768/m768 (AB)
                  foxh1ty68b/ty68b
                  ndr2tf219/tf219
                  noton1/n1 (AB)
                  sox32ta56/ta56
                  tbx16b104/b104 (AB)
                  tbxtab195/b195
                  tdgf1m134/m134 (AB)
                  WT
                  WT + MO1-dnah9
                  1 - 10 of 11
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                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  No data available
                  Mapping
                  No data available
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                  Citation
                  Essner, J.J., Amack, J.D., Nyholm, M.K., Harris, E.B., and Yost, H.J. (2005) Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut. Development (Cambridge, England). 132(6):1247-1260.