Morpholino

MO3-disc1

ID

ZDB-MRPHLNO-090825-1

Name

MO3-disc1

Previous Names

MOE3 (1)

Target

disc1 (1)

Sequence

5' - GGACTCGATCTAATGAGAGAAATCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

designed for the intron 2/exon 3 splice-acceptor site to alter splicing of the disc1 pre-mRNA, deleting exon 3, which introduces a premature stop site and a severely truncated protein.

Genome Resources

None

Target Location

Genomic Features

No data available

Expression

Gene expression in Wild Types + MO3-disc1

Expressed Gene	Anatomy	Figures
foxd3	cranial neural crest posterior lateral line ganglion glial cell trigeminal ganglion glial cell	Fig. 3 , Fig. 8 from Drerup et al., 2009
phox2bb	epibranchial ganglion	Fig. 2 from Culbertson et al., 2011
sox10	cranial neural crest	Fig. 3 from Drerup et al., 2009

Phenotype

Phenotype resulting from MO3-disc1

Phenotype	Fish	Figures
cartilage morphogenesis decreased process quality, abnormal	WT + MO3-disc1	Fig. 2 from Culbertson et al., 2011
ceratobranchial cartilage absent, abnormal	WT + MO3-disc1	Fig. 2 from Culbertson et al., 2011
ceratohyal cartilage malformed, abnormal	WT + MO3-disc1	Fig. 2 from Culbertson et al., 2011
cranial cartilage malformed, abnormal	WT + MO3-disc1	Fig. 2 from Drerup et al., 2009
cranial neural crest increased size, abnormal	WT + MO3-disc1	Fig. 3 from Drerup et al., 2009
Meckel's cartilage malformed, abnormal	WT + MO3-disc1	Fig. 2 from Culbertson et al., 2011
neural crest cell migration disrupted, abnormal	ba2Tg/+ + MO3-disc1	Fig. 3 , Fig. 4 , Fig. 5 from Drerup et al., 2009
palatoquadrate cartilage malformed, abnormal	WT + MO3-disc1	Fig. 2 from Culbertson et al., 2011
posterior lateral line ganglion glial cell increased amount, abnormal	WT + MO3-disc1	Fig. 8 from Drerup et al., 2009
trigeminal ganglion glial cell increased amount, abnormal	WT + MO3-disc1	Fig. 8 from Drerup et al., 2009
vagal ganglion malformed, abnormal	WT + MO3-disc1	Fig. 2 from Culbertson et al., 2011

Phenotype of all Fish created by or utilizing MO3-disc1

Phenotype	Fish	Conditions	Figures
posterior lateral line ganglion glial cell increased amount, abnormal	WT + MO3-disc1	standard conditions	Fig. 8 from Drerup et al., 2009
cranial neural crest increased size, abnormal	WT + MO3-disc1	standard conditions	Fig. 3 from Drerup et al., 2009
cranial cartilage malformed, abnormal	WT + MO3-disc1	standard conditions	Fig. 2 from Drerup et al., 2009
ceratohyal cartilage malformed, abnormal	WT + MO3-disc1	standard conditions	Fig. 2 from Culbertson et al., 2011
ceratobranchial cartilage absent, abnormal	WT + MO3-disc1	standard conditions	Fig. 2 from Culbertson et al., 2011
palatoquadrate cartilage malformed, abnormal	WT + MO3-disc1	standard conditions	Fig. 2 from Culbertson et al., 2011
vagal ganglion malformed, abnormal	WT + MO3-disc1	standard conditions	Fig. 2 from Culbertson et al., 2011
neural crest cell migration disrupted, abnormal	WT + MO3-disc1	standard conditions	Fig. 3 from Drerup et al., 2009
cartilage morphogenesis decreased process quality, abnormal	WT + MO3-disc1	standard conditions	Fig. 2 from Culbertson et al., 2011
trigeminal ganglion glial cell increased amount, abnormal	WT + MO3-disc1	standard conditions	Fig. 8 from Drerup et al., 2009
Meckel's cartilage malformed, abnormal	WT + MO3-disc1	standard conditions	Fig. 2 from Culbertson et al., 2011
neural crest cell migration disrupted, abnormal	ba2Tg/+ + MO3-disc1	standard conditions	Fig. 4 , Fig. 5 from Drerup et al., 2009

Citations