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Fig. 1 Comparison of the effects of wildtype and hcp2 mutant strains on virulence and inflammatory activation in zebrafish larvae. (A-B) Phenotypes and their ratios of zebrafish larvae infected with wildtype V. alginolyticus and hcp2 mutant strains. Greater transparency in the ventral region of the yolk sac and intestine was indicative of more severe edema in zebrafish. (C) Transcriptional levels of innate immune receptors tlr2, tlr4, and tlr5 in zebrafish infected with the wildtype and the hcp2 mutant strains. (D) Transcriptional analysis of inflammatory factors il6, il10, il12, and tnf-α in zebrafish infected with wildtype and hcp2 mutant strains. Scale bars: 500 μm in Figure 1A, n: number of analyzed zebrafish larvae, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significance; tnf-α, tumor necrosis factor-α.