Fig. 6

Fig. 6 Gck:GFP expression in the islet in pdx1^-/- diabetic larvae is reduced.

a, bgck:GFP and ins:dsRed labeled cells in the islets of 6 and 10 dpf control and pdx1^-/- after MIN and HFD diet. Scale bar 50 µm. c, d Relative quantification of gck:GFP expressing cells and ins:dsRed labeled β-cells in the islet of pdx1^-/- compared to controls at 6 dpf. Dot plots with indicated mean. Statistical significance assessed by t-test. e, f Relative quantification of gck:GFP expressing cells and ins:dsRed labeled β-cells in the islet of pdx1^-/- compared to controls at 10 dpf. Dot plots with indicated mean. Statistical significance assessed by Two-way ANOVA, P-values of multiple comparisons are indicated. gInsulin gene expression levels analyzed via RT-qPCR under MIN and HFD feeding (N > 3). Dot and bar plot shows mean with SD. Statistical significance assessed by Two-way ANOVA. P-values of multiple comparisons are indicated.