ZFIN Image: Li et al., 2022, FIGURE 1

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FIGURE 1

RT-PCR and qRT-PCR analysis of candidate pluripotency genes in 10 stages of em[bryonic development of Zebrafish (ZF) and Crucian carp (CC). (A) RT-PCR analysis of 16 pluripotency-related genes in 10 stages of embryonic development of zebrafish. (B) RT-PCR analysis of eight pluripotency-related genes in 10 stages of embryonic development of crucian carp. (C) qRT-PCR analysis for candidate pluripotency genes (Nanog, Oct4, Tdgf1, Gdf3 and Klf17) through the 10 stages of ZF and CC embryos. It was clearly showed that these four genes were seemed to be mainly expressed in the pluripotent cells of undifferentiated embryos, but the expression was significantly decreased or even lost after embryonic cell differentiation. For each gene detected, the △CT value was calculated from the average CT value of the independent housekeeping gene β-actin. For each sample, At least three independent experiments were done for these results.

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Acknowledgments

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