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Fig. 1 (A) Post-migratory NCCs are double transgenic (fli1a:EGFP;sox10:mRFP) and reside in the frontonasal region and in the anterior and posterior pharyngeal arches. Double transgenic animals were dissociated into single-cell suspension. (B) Scatter plot from FACS. Dashed box indicates double-positive cells sorted for single-cell transcriptomics. (C) scRNA-seq clusters double-transgenic NCCs into four distinct populations. (D-F) Genes enriched in each cluster indicate population identity. Example plots for the genes shown in bold are displayed. All individual feature plots have the same scale indicated in D.

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