FIGURE

Figure 5

ID

ZDB-FIG-260820-46

Publication

Vu et al., 2026 - G protein‑coupled receptor kinase 4 governs early kidney development via STAT3 and Nhe3a

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Figure 5

The actions of Grk4 during kidney development do not rely on its nuclear localization. (A) Confocal sections of 293T cells transiently transfected with GFP-tagged human GRK4 and GRK4 ΔNLS. Scale bar, 10 μm. (B) Both wild-type GRK4 and GRK4 ΔNLS restored normal nhe3a expression in Grk4-depleted embryos. Scale bar, 200 μm. (C) Analysis of the length of the nhe3a-positive pronephric tubule. n=4 experiments with 71-115 embryos in total. ^*P=0.0147 and ^****P<0.0001 (data were analyzed using the Kruskal-Wallis-test with Dunn's multiple comparison test). (D) Wild-type GRK4 and GRK4 ΔNLS rescue hydrocephalus formation in Grk4 MO-injected embryos. n=6 experiments with 161-175 embryos in total. ^****P<0.0001 (data were analyzed using a two-sided Fisher's exact test with the Bonferroni correction applied). Scale bar, 200 μm. (E) Glomerular cyst formation in Grk4 LOF embryos is reduced by wild-type GRK4 and GRK4 ΔNLS. n=8 experiments with 173-217 embryos in total. ^****P<0.0001 (data were analyzed using a two-sided Fisher's exact test with the Bonferroni correction applied). Scale bar, 100 μm. Grk4, G protein-coupled receptor kinase 4; NLS, nuclear localization sequence; CTRL, control; MO, morpholino oligonucleotide; wt, wild-type.

Expression Data

Gene:	slc9a3.1
Fish:	WT WT + MO3-grk4
Knockdown Reagent:	MO3-grk4
Anatomical Term:	pronephric tubule
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-grk4
Knockdown Reagent:	MO3-grk4
Observed In:	brain pronephric glomerulus pronephric tubule
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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