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Gper1 role in chronic skin inflammation is related to the regulation of keratinocyte proliferation. a Schematic of the experimental procedure used. Eggs at one-cell stage were microinjected with CRISPR-Cas9 system, using gper1 or control gRNA. At 2 dpf, a BrdU assay was performed. b Representative images of BrdU assay in 2 dpf control and Gper1-deficient larvae. c Quantification of BrdU+ cells per area in skin in both conditions. d Schematic of the experimental procedure used. Spint1a-deficient one-cell stage eggs were microinjected with the CRISPR-Cas9 system, using gper1 or control gRNA. Treatments of interest were added to dechorionated embryos at 1 dpf by bath immersion and renewed daily. At 3 dpf, images were taken to analyze keratinocyte aggregates and neutrophil distribution. e Representative images of 3 dpf control and Gper1-deficient larvae, treated with Palbociblib (Palbo) or vehicle (DMSO). f Comparison of the number of KC aggregates in tail skin between non-edited and Gper1-deficient larvae, control or treated. g Quantification of neutrophil distribution in the tail in each group. Each dot represents one individual, and the means and SEM for each group are also shown. P values were calculated by Student’s t test in graphs (b, d) and by One-way ANOVA in graphs (f, g). ns not significant; *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001.
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