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Genetic inhibition of gper1 aggravates skin alterations and neutrophil infiltration in Spint1a-deficient larvae. a Schematic of the experimental procedure used. Eggs at one-cell stage were microinjected with CRISPR-Cas9 system, using gper1 or control gRNA. At 3 dpf, images were taken to analyze KC aggregates and neutrophil distribution. b Analysis of genome editing efficiency in larvae injected with gper1 gRNA/Cas9 complexes and quantification rate of nonhomologous end joining mediated repair, showing all insertions and deletions at the target site using TIDE (https://tide.nki.nl). c Representative images of 3 dpf control and Gper1-deficient larvae. d Quantification of the number of skin KC aggregates in control and Gper1-deficient larvae. e Quantification of neutrophil distribution in the tail in control and Gper1-deficient larvae. Each dot represents one individual, and the means and SEM for each group are also shown. P values were calculated by Student’s t test. ***P ≤ 0.001; ****P ≤ 0.0001.
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