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Genetic inhibition of gper1 did not affect inflammatory markers in Spin1a-deficient larvae. a Schematic of the experimental procedure used. Spint1a-deficient one-cell stage eggs were microinjected with CRISPR-Cas9 system, using gper1 or control gRNA. At 2 dpf, TUNEL assay was performed. At 3 dpf, images were taken to analyze Nfkb/Il1b reporter expression or ROS fluorescence. b Representative images of 3 dpf control and Gper1-deficient NFkB:GFP+ larvae. c Quantification of Nfkb activity in skin in each group. d Representative images of 3 dpf control and Gper1-deficient il1b:GFP+ larvae. e Quantification of Il1b expression in skin in every condition. f Representative images of 3 dpf control and Gper1-deficient larvae, incubated with H2O2 fluorescent probe. g Quantification of skin H2O2 production in every group. h Representative images of 2 dpf control and Gper1-deficient larvae. i Number of TUNEL+ cells in skin in each condition. Each dot represents one individual, and the means and SEM for each group are also shown. P values were calculated by Student’s t test when parametric (Graphs c, g, i) or Mann–Whitney test when no parametric (Graph e). ns, not significant.
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