Whole-mount in situ hybridization (WISH) for c-Fos before and after social interaction in the ube3a mutants and WT. a Dorsal and lateral views of adult ube3a WT zebrafish before social interaction. b Dorsal and lateral views of adult ube3a WT zebrafish after social interaction. c Dorsal and lateral views of adult ube3a mutant zebrafish before social interaction. d Dorsal and lateral views of adult ube3a mutant zebrafish after social interaction. e Relative intensity of c-Fos signal expression in different brain regions (Lca, Va, Cce, TeO, Dm, Hyp, PPa and PPp) before and after social experience for WT and mutants (N = 3, multiple unpaired t tests and correction via the Holm‒Sidak method). Va valvular cerebelli, Dm medial zone of the dorsal telencephalic area, Cce corpus cerebelli, TeO optic tectum, Lca locus caudalis, Hyp hypothalamus, PPa parvocellular preoptic nucleus (anterior part), PPp parvocellular preoptic nucleus (posterior part). The data are presented as the mean ± SEM. ns: non-significant, *P < 0.05.
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