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Characterization of the ube3a mutant line. a Structure of the zebrafish ube3a gene. The mutation induces a stop codon at exon 3/11, with a substitution of T > A. The structure was obtained from Ensembl (ENSDART00000078128). b Prediction of the mutated protein structure. Created with SwissModel (https://swissmodel.expasy.org/interactive). c Genotyping results showing the WT and mutant alleles. d The expression level of ube3a mRNA in WT and mutant’ adult brains (N = 3; unpaired t test). e Left: Western blot analysis of the WT and mutant adult brains. Right: Quantification of the relative intensity of ube3a normalized to that of β-Actin (N = 3, unpaired t test). f Comparison of the physical appearance of the WT and mutant zebrafish (N = 6; unpaired t test). g Hatching rates of WT and the ube3a mutant from 48 hours postfertilization (hpf) to 60 hpf. h Survival rates of WT and mutant ube3a zebrafish. The data are shown as the mean ± S.E.M. ns: not significant, **P < 0.01.
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