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Dancr regulates gene expression pathways involved in cell death. CRISPR-Cas9 mediated deletion of the zebrafish dancr promoter leads to a reduction in dancr, snora26 and snora27 expression. (A) Integrative Genomics Viewer visualisation of RNA-seq reads mapping to the dancr locus in mock control and F0 dancr crispant embryos at 30 hpf. The locations of the four guide RNAs targeting the dancr promoter and primers used for RT-qPCR are shown. (B)Dancr, snora26 and snora27 expression levels were measured in F0 dancr crispant and mock control embryos at 30 hpf using RT-qPCR. actb2 was used as a reference gene. Results presented as mean ± sem, n = 3. Two-tailed two sample t-test p < 0.01**. Individual dots represent separate biological replicates. (C, D)Dancr regulates gene pathways involved in cell death and the immune response in the developing zebrafish embryo. (C)Dancr promoter deletion induces statistically significant changes in the expression of 164 genes (DESeq2 padj<=0.05; log2FoldChange>=1.0). (D) KEGG pathway analysis of the dancr regulated gene set identified significantly enriched pathways (FDR 5%). The number of genes found in each category are indicated.
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