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The habenula provides landmark information to the IPN. a An example plane showing correlation values of IPN cells with light in a particular position before and after NFP treatment for an Ntr+ (top) and a control (bottom) fish. b Maximum correlation maps for all fish in the dataset. In each panel, each pixel shows the maximum absolute correlation value across all fish, planes and landmark positions (n(Ntr + )=8, n(Ntr-)=8). c Left, distribution of correlation values of IPN neurons with the RF regressors before (blue) and after (purple) NFP treatment for Tg(16715:Gal4; UAS:Ntr-mCherry) fish (habenula ablated group, mean ± sd, n(fish)=8). The variance of the pre-ablation distribution is significantly larger than the variance of the post ablation distribution (Wilcoxon signed-rank test, p = 0.004). Right, distribution of correlation values of IPN neurons with the sensory regressors before (blue) and after (purple) NFP treatment for Ntr-fish (control group, mean ± sd, n(fish)=8). The variance of the pre-ablation distribution is not significantly larger than the post ablation (one-sided Wilcoxon signed-rank test, p = 0.9). d Number of reliably tuned neurons in the IPN before and after NFP treatment for Ntr+ (left) and control (right) fish. NFP significantly reduces the number of responsive cells in the IPN in the Ntr+ group (Wilcoxon signed-rank test, p = 0.004, n(fish)=8) but not in the control group (one-sided Wilcoxon signed-rank test, p = 0.87, n(fish)=8). Cell count was done by counting all reliably responding neurons in the IPN (neurons with a reliability threshold > 0.25).
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