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Whole brain responses to whole field visual motion. a Illustration of experimental design. Left, side view of an embedded zebrafish larva under the microscope. Center, top view of embedded zebrafish larva in a lightsheet imaging chamber. Agarose is removed from the tail to allow swimming and tail tracking. Agarose is also removed from the side and front to prevent the laser from scattering. Right, a zebrafish on top of a pink noise pattern moving in 8 possible directions. b horizontal and sagittal views of the zebrafish brain (MapZeBrain atlas reference brain). Lines point to specific regions that were found to respond to whole field visual motion. PT: pretectum, OT: optic tectum, aHB: anterior hindbrain, IPN: interpeduncular nucleus. c Three views of all ROIs extracted from a whole brain dataset (one example fish). Neurons are colored according to their correlation value with rightward motion. d Three views of the same fish, only reliably responding neurons are shown (top 5% of reliability index). Each neuron is colored according to the direction it is tuned to. e Same as d but for all fish in the dataset, registered to a reference fish (n = 15). f Example neurons that are tuned to different directions. Left, tuning curves of 8 neurons. Right, the full traces from the entire experiment for the same neurons. The colorful shadings indicate the direction that is being presented. g Distributions of correlation values with each direction for neurons in the left habenula (LHab), IPN, aHB and pretectum (n = 15).
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