Fig. 3
- ID
- ZDB-FIG-251003-33
- Publication
- Lemée et al., 2025 - Disrupted transcriptional networks regulated by CHD1L during neurodevelopment underlie the mirrored neuroanatomical and growth phenotypes of the 1q21.1 copy number variant
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Dosage changes of Chd1l control neuronal production in transient mouse model. (A) Schematic of in utero electroporation. Mouse embryos are electroporated at E13.5, mouse brain are dissected at E14.5 and stained for Tbr1 neuronal marker. Double GFP-positive and Tbr1-positive cells representing the newborn neurons are counted in a defined area in the VZ/SVZ. (B) E14.5 mouse brain slices for control and Chd1l overexpressant conditions. (C) Dot plot showing the percentage of Tbr1-positive cells among the electroporated GFP-positive cells from the defined area in E14.5 mouse brains from control or electroporated with Chd1l expression vector. Data shown as mean ± SEM, electroporated and imaged embryos were from five different litters for control condition and from six different litters for Chd1l overexpression condition; Student’s t-test. (D) E14.5 mouse brain slices for control and knockdown Chd1l conditions. (E) Dot plot showing the percentage of Tbr1-positive cells among the electroporated GFP-positive cells in E14.5 mouse brains from control or electroporated with esiChd1l RNA. Data shown as mean ± SEM, electroporated and imaged embryos were from three different litters for esiRluc condition and from four different litters for esiChd1l condition; Student’s t-test. CP, cortical plate ; IZ, intermediate zone ; VZ/SVZ, ventricular/subventricular zones. Scale bars: 50 μm. |