Fig. 6

Gmfb enhances il6/stat3 activation and actin-filaments distribution in zebrafish hepatocytes. A: expression levels of proteins p-stat3, stat3, p-jnk, jnk, p-erk, erk, gmfb, and gapdh normalization control in liver samples from PHX and MASLD models of WT, HepGKO, and HepGOE zebrafish. n = 6 samples, one fish per sample for PHX model and 10 fish per sample for MASLD model. Data were presented as relative expression levels. One-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. ND, BC, or WT group. B: expression levels of proteins p-stat3, stat3, and gapdh normalization control in zebrafish primary hepatocytes treated with BSA, EGF, EGF plus IL6, or EGF plus HGF plus IL6, respectively. n = 3 samples per group, 8 fish per sample. Data were presented as relative expression levels. One-way ANOVA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. BSA or WT group. C: representative images of phalloidin stained (red) F-actin in liver slices from ND and HFC groups of WT, HepGKO, and HepGOE zebrafish. Scale bar = 20 μm. n = 8 samples per group, one fish per sample. D: representative images of phalloidin stained (red) F-actin in 24 h post-seeding primary hepatocytes isolated from WT, HepGKO, and HepGOE zebrafish. Scale bar = 5 μm. n = 3 samples per group, 8 fish per sample. E: quantification of phalloidin (red) fluorescence intensity in C and D. One-way ANOVA, *P < 0.05, ***P < 0.001, ****P < 0.0001 vs. ND or WT group. Experiments were repeated on 3 clutches. BC, baseline control; BSA, bovine serum albumin; EGF, epidermal growth factor; erk, extracellularly regulated kinase; gapdh, glyceraldehyde-3-phosphate dehydrogenase; gmfb, glia maturation factor-β; hep-gmfb, gmfb in hepatocyte; HepGKO, hepatocyte-specific gmfb knockout; HepGOE, hepatocyte-specific gmfb overexpression; HFC, high fat, high cholesterol diet; HGF, hepatocyte growth factor; IL6, interleukine 6; jnk, c-jun N-terminal kinase; MASLD, metabolism-associated steatotic liver disease; ND, normal diet; stat3, signal transducer and activator of transcription 3; WT, wild-type.