FIGURE

Fig. 4

ID

ZDB-FIG-250804-4

Publication

Wen et al., 2025 - The Neuroprotective Effects of the Crinoid Natural Compound Rhodoptilometrin in Parkinson's Disease Experimental Models: Implications for ER Stress and Autophagy Modulation

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Fig. 4

Impact of inhibiting autophagy on the neuroprotective activities of RDM in SH-SY5Y cells treated with 6-OHDA. (A) Experimental flowchart. On day 1, cells were plated. On day 2, cells were pretreated with RDM for 1 h followed by treatment with 3-methyladenine (3-MA), an autophagy inhibitor. The cells were then subjected to 6-OHDA-induced neurotoxicity for 16 h. Cell viability was assessed using alamarBlue staining. (B) 3-MA cytotoxicity on SH-SY5Y cells using alamarBlue assay. Cells were treated with 3-MA at concentrations ranging from 1 to 100 μM for 16 h. The relative viability of control cells is presented as 100% for normalization. (C) Assessment of neuroprotective activity with 3-MA (10 μM) and RDM (10 μM) treatment. The relative neuroprotection (relative protection) was calculated based on cell viability, with the relative cell protection of untreated cells presented as 0%. *p < 0.05 compared with control group; #p < 0.05 compared with 6-OHDA alone group. RDM, rhodoptilometrin.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ ACS Chem. Neurosci.