FIGURE

Fig. 4

ID
ZDB-FIG-250710-64
Publication
D'Silva et al., 2025 - Dis3l2 is essential for neural crest survival by modulating Akt signaling
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

dis3l2 is required for early embryonic mitoses A: Sum projection confocal image of 256 cell stage control and dis3l2 morphants embryos showing mitotic defects. Blue/DAPI - DNA, red– alpha-tubulin, green– γ tubulin. B: Enlarged sum projection confocal image of dis3l2 morphants showing cytokinetic index (white arrowhead), spindle length (white dotted line), and spindle poles (white asterisks) during early mitoses. Scale bars, 50 μm. C: Quantification of spindle length (white dotted line), chromosome congression, spindle pole (white asterisk), and cytokinesis (white arrowhead) in the dis3l2 morphants as compared to control. Scale bars, 50 μm. All data are shown as mean + SEM. *p < 0.05, **p < 0.01, ***p < 0.001. n = 3 for each experiment, with a minimum of 20 embryos per experiment. D: Relative expression of cell cycle regulators– p21, p27, and cyclinD1 in dis3l2 morphants. Data are shown as mean + SEM, *p < 0.05, **p < 0.01, ***p < 0.001. n = 3 for each experiment, with a minimum of 100 embryos per experiment. E: Western blot analysis showing Cdk1 and Mek1 levels in dis3l2-depleted embryos as compared to control. Gapdh was used as the loading control

Expression Data
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 256-cell

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage: 256-cell

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Commun. Signal.