FIGURE

Fig. 4.

ID

ZDB-FIG-250702-76

Publication

Oikemus et al., 2025 - Identifying optimal conditions for precise knock-in of exogenous DNA into the zebrafish genome

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Fig. 4.

Homology-matched HDR template improves precise knock-in rates. (A) Indel rates with Cas9 RNP alone (no tem), or co-injected with HDR templates targeting the h3f3d ATG that were PCR-amplified with vector (vecPCR) or homology-matched (homPCR) primers. (B-F) Frequencies of targeted insertion of indicated repair event using vector or homology-matched template at the h3f3d locus. P-values are indicated (unpaired t-test). Error bars represent s.d.; n=3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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