FIGURE

Fig. 6

ID

ZDB-FIG-250527-6

Publication

Saquet et al., 2024 - Knockout of rbm24a and rbm24b genes in zebrafish impairs skeletal and cardiac muscle integrity and function during development

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Fig. 6

Disrupted integrity and function of skeletal muscles in rbm24a and rbm24b mutants. (A–C″) Lateral views, anterior to the left, showing the muscle tissue. Scale bar: 250 μm. (D) Graph representing the average normalized area of skeletal muscle pixel intensities (mean gray values) shows the reduction of trunk muscle tissues in single and double mutants. At each developmental stage, 15 to 20 embryos per genotype were analyzed. (E–F″) Lateral views comparing myofiber organization in rbm24a single mutants and rbm24a−/−;rbm24b−/− double mutants. Scale bars: 25 μm. (G, G') Representative TEM images of skeletal muscle sections at 3 dpf show that the dark A-band and the light I-band patterning is severely disrupted by accumulation of disordered sarcoplasmic reticulum (black arrow) in double mutants (n = 4). Scale bars: 1 μm. (H) Graph comparing the velocity of rbm24a single mutants and rbm24a−/−;rbm24b−/− double mutants at 2, 3 and 4 dpf. Average velocity with standard error was plotted and significant deviation was determined using the Student's t test. At each developmental stage, 15 to 20 embryos per genotype were analyzed

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rbm24a^sdu28/sdu28 rbm24a^sdu28/sdu28; rbm24b^sdu29/sdu29
Observed In:	muscle cell skeletal muscle swimming
Stage Range:	Long-pec to Day 4

Phenotype Detail

Acknowledgments

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