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General progress of ossification in craniofacial and axial skeletal elements. (A) Ossification pattern and progress shows no statistically significant differences between Alizarin Red stained larvae of the three genotypes. The images show homozygous mutants (mut) and wildtype (wt) viewed from laterally and ventrally. (B) Formation of chordacentra in the zebrafish column. The overall number of vertebrae is without any statistically significant differences, indicating that the onset of chordacentra ossification is not affected by the mutation. Ossification seems to progress faster in wildtype larvae, as confirmed by statistical analysis. Anterior to the left, dorsal up. Abbreviations: ns, not significant; * significant; wt, wildtype; mut, homozygous mutant; het, heterozygous mutant. Black circles and triangles indicate developed and developing vertebrae, respectively. Scale bars: 50 µm in (A), 500 µm in (B).
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