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Morphometric analysis of Standard length (SL) and head length and width. Comparison of Alcian Blue stained wild type versus mutant larvae at 12 dpf. (A) SL in mm was determined using a microscopic ruler implementing 100 µm increments. Anterior to the left, lateral view and dorsal up. Head length (B) and width (C) in all larvae was measured, as depicted in the schematics, from images of the basal neurocranium using imageJ software (version 1.51h; National Institutes of Health, Bethesda, MD, USA). n = All: anterior to the left. (A) lateral view; (B,C) ventral view. Abbreviations: ns, not significant; wt, wildtype; mut, homozygous mutant; het, heterozygous mutant. Scale bars: 200 µm, except in (A): 1000 µm.
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