FIGURE

Fig. 4

ID
ZDB-FIG-250428-161
Publication
Gong et al., 2024 - Transient receptor potential a1b regulates primordial germ cell numbers and sex differentiation in developing zebrafish
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Fig. 4

Analysis of differentially expressed genes (DEGs) in Danio rerio. (a) Schematic representation of tissue sampling near the genital ridge in zebrafish at 1.25 days fertilization (dpf) for RNA sequencing (RNA-seq) analysis. (b) Volcano plot depicting DEGs in wild-type (WT) and trpa1b homozygous zebrafish at 1.25 dpf, with 905 upregulated DEGs and 745 downregulated DEGs. (c) Gene ontology (GO) term and KEGG pathway analysis of DEGs in the vicinity of the genital ridge in WT and trpa1b−/− zebrafish, annotated for biological processes (in red), molecular function (in blue), and cellular components (in green) with a p-value cutoff of 0.05. (d) At 1.25 dpf, there is low expression of PGC formation-related genes nanos3 and ddx4, and high expression of the tdrd6 gene. (e) At 1.25 dpf, there is low expression of genes related to spermatid development and differentiation, such as sycp3, and high expression of cyp11c1, amh, and pla2g4aa. *p < 0.05; **p < 0.01; ***p < 0.001.

Expression Data
Genes:
Fish:
Anatomical Term:
Stage: Prim-15

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Prim-15

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Fish Biol.