FIGURE

Fig. 4

ID

ZDB-FIG-250428-161

Publication

Gong et al., 2024 - Transient receptor potential a1b regulates primordial germ cell numbers and sex differentiation in developing zebrafish

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Fig. 4

Analysis of differentially expressed genes (DEGs) in Danio rerio. (a) Schematic representation of tissue sampling near the genital ridge in zebrafish at 1.25 days fertilization (dpf) for RNA sequencing (RNA-seq) analysis. (b) Volcano plot depicting DEGs in wild-type (WT) and trpa1b homozygous zebrafish at 1.25 dpf, with 905 upregulated DEGs and 745 downregulated DEGs. (c) Gene ontology (GO) term and KEGG pathway analysis of DEGs in the vicinity of the genital ridge in WT and trpa1b−/− zebrafish, annotated for biological processes (in red), molecular function (in blue), and cellular components (in green) with a p-value cutoff of 0.05. (d) At 1.25 dpf, there is low expression of PGC formation-related genes nanos3 and ddx4, and high expression of the tdrd6 gene. (e) At 1.25 dpf, there is low expression of genes related to spermatid development and differentiation, such as sycp3, and high expression of cyp11c1, amh, and pla2g4aa. *p < 0.05; **p < 0.01; ***p < 0.001.

Expression Data

Genes:	amh cyp11c1 ddx4 nanos3 pla2g4aa sycp3 tdrd6a
Fish:	AB trpa1b^sou18/sou18 (AB)
Anatomical Term:	whole organism
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	trpa1b^sou18/sou18 (AB)
Observed In:	whole organism
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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