FIGURE

Fig. 1

ID

ZDB-FIG-250428-158

Publication

Gong et al., 2024 - Transient receptor potential a1b regulates primordial germ cell numbers and sex differentiation in developing zebrafish

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Fig. 1

Targeted disruption of the zebrafish trpa1b gene using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9. (a) Structure of the zebrafish trpa1b gene and target site. The mutant site contains a − 3 bp (ATG) DNA sequence and a + 9 bp (TCCGCTGAC) insertion sequence, which includes a stop codon. (b) Schematic representation of the wild-type (WT) and Trpa1b proteins. Functional domains are indicated in red and consist of amino acids (aa) from 760 to 976, which do not reach the ion channel domain. (c) Detection of Trpa1b protein expression at 60 h postfertilization (hpf). KO: Trpa1b−/−. (d) Whole-embryo in situ hybridization showing the temporal expression of trpa1b. (e–f) Analyse the relative expression levels of trpa1b messenger RNA (mRNA) in different adult tissues and embryonic development stages using real-time quantitative PCR (RT-qPCR). The data are presented as mean ± SE of mean (n = 3, biological replicates).

Expression Data

Gene:	trpa1b
Antibody:	Ab1-trpa1b
Fish:	AB trpa1b^sou18/sou18 (AB)
Anatomical Terms:	brain epiblast eye fin gill head heart integument intestine kidney liver muscle ovary testis whole organism
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	trpa1b^sou18/sou18 (AB)
Observed In:	whole organism
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Fish Biol.