Escape response of WT and NaVDKO zebrafish. (A) Snapshots of the representative escape responses of WT (upper) and NaVDKO (lower) zebrafish at 4 dpf in normal water. Images were captured every 2 ms. Scale bar: 1 mm. (B) Representative plots of the “neck angle” during the escape behavior of WT and NaVDKO fish. The inset shows the definition of “neck angle.” (C) Analysis of the maximum neck angle in the first turn of 4 dpf fish. Individual data points are shown as dots. Bars and error bars represent the mean and SEM, respectively (n = 10 for WT, n = 9 for NaVDKO). (D) Analysis of the maximum turn angle speed of 4 dpf fish. The maximum turn angle speed was calculated from the differential of the neck angle (n = 10 for WT, n = 9 for NaVDKO). The numerical data presented in this figure can be found in S1 Data.
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