FIGURE

Fig. 6

ID

ZDB-FIG-250410-19

Publication

Liu et al., 2025 - Knockout of the fcsk gene in zebrafish causes neurodevelopmental defects

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Fig. 6

GDP-L-fucose and GFP-tagged human FCSK rescue early development and fucosylated protein levels in fcsk−/− zebrafish A: Body length and statistical analysis of WT (injected with H2O), WT (injected with GDP-L-fucose), fcsk−/− (injected with H2O), and fcsk−/− (injected with GDP-L-fucose) zebrafish (5 dpf, n=24). Scale bar: 200 µm. B: Western and lectin blot analyses of WT (injected with H2O), WT (injected with GDP-L-fucose), fcsk−/− (injected with H2O), and fcsk−/− (injected with GDP-L-fucose) zebrafish (48 hpf). C: Body length and statistical analysis of WT (injected with pEGFP-C1), WT (injected with GFP-tagged human FCSK), fcsk−/− (injected with pEGFP-C1), and fcsk−/− (injected with GFP-tagged human FCSK) zebrafish (5 dpf, n=19). Scale bar: 200 µm. D: Western and lectin blot analyses of WT (injected with pEGFP-C1), WT (injected with GFP-tagged human FCSK), fcsk−/− (injected with pEGFP-C1), and fcsk−/− (injected with GFP-tagged human FCSK) zebrafish (72 hpf). Mean±SEM. ns: Not significant; *: P<0.05; ***: P<0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	fcsk^{hzu301/hzu301} (AB)
Condition:	chemical treatment by injection: GDP-L-fucose
Observed In:	whole organism whole organism protein O-linked fucosylation
Stage Range:	Long-pec to Day 5

Phenotype Detail

Acknowledgments

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