Bivalent chromosome analysis and sperm ploidy detection of WT and cdk1+/− zebrafish. A Ploidy detection of sperms of WT zebrafish. B Ploidy detection of sperms of cdk1+/− zebrafish. C Assessment of sperm morphology of WT and cdk1+/− zebrafish. The black arrows indicated large-head sperms. Scale bar = 5 µm. D Measurement of sperm head of WT and cdk1+/− zebrafish. E Observations on the bivalent chromosome of WT zebrafish. Scale bar = 5 µm. F Observations on the bivalent chromosome of cdk1+/− zebrafish. Scale bar = 5 µm. cdk1, cyclin-dependent kinase 1; WT, wild-type zebrafish; cdk1+/−, zebrafish with heterozygous mutation of cdk1; n, number; 1N, haploid; 2N, diploid; 3N, triploid; 4N, tetraploid. ***p < 0.001 indicated an extremely significant difference
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