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Reduction in trpv6+ NaR cell number in the sox10 mutants. (A) Immunostaining of a 3 dpf SOX10:Cre; ef1a: DsRed>GFP larva with an antibody against the Na+/K+ ATPase pump confirms that ionocytes do not derive from neural crest. (B) rt-PCR demonstrates that trpv6 transcription is not overtly altered at the whole-body level at 4 dpf. Each point represents a pool of 10-15 embryos (unpaired t-test; P=0.954, d.f.=4). (C-D′) Fluorescence in situ hybridizations for trpv6 (C) and igfbp5a (D) demonstrate a striking and significant reduction in NaR cell number in mutants at 4 dpf (quantified in C′,D′), with partial recovery by 7 dpf (unpaired t-tests; trpv6: 4 dpf: P<0.000001, d.f.=20; 7 dpf: P=0.0006, d.f.=17; igfbp5a: 4 dpf: P=0.0001, d.f.=23; 7 dpf: P<0.0001, d.f.=17). In B,C′,D′, dashed lines indicate the median. ns, not significant; WT, wild type. Scale bars: 100 µm.
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