FIGURE

Fig. 3

ID
ZDB-FIG-250130-58
Publication
Zhang et al., 2024 - Integrative mRNA and miRNA Expression Profiles from Developing Zebrafish Head Highlight Brain-Preference Genes and Regulatory Networks
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Fig. 3

Snap25b knockout causes embryonic development defects and decreases swimming activity. The snap25b gene structure and the schema for CRISPR-Cas9 technology to reduce snap25b expression in zebrafish. B Sanger sequencing confirms the mutations caused by CRIPSR-Cas9 in F0 and F1 generations. Primary sequence was shown. C In situ hybridization of snap25b in wild-type (WT) and snap25b mutant zebrafish. D The expression of snap25b by RT-qPCR in the head of wild-type and snap25b mutant zebrafish. E The representative abnormal phenotype included developmental delay, curled tail, and agenesis of eye. F The summary of survival rate in wild-type and snap25b mutants. G The summary of abnormality rate in wild-type and snap25b mutants. The Danio Vision system recorded the swimming behavior of wild-type and snap25b mutants (H) and analysis of total swimming distance (I). Data are presented as mean ± SD, n = 3 or n = 15–18; ** p < 0.01

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurobiol.