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Fig.4

ID

ZDB-FIG-250122-63

Publication

Ranard et al., 2024 - Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment

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Fig.4

Generation of elovl2 mutant zebrafish with CRISPR/Cas9 genome editing. A: Schematic of the elovl2 gene and gRNA sequences, identified as targets A and B. B: Genomic sequences for wild-type elovl2 and one of the two generated mutant alleles, elovl2^co100. Sequence peak tracings and PCR verification are shown in Supplemental Fig. 2. The elovl2^co100 allele has a 157 bp deletion (107 bp in exon 3; 50 bp in intron 3) and 13 bp insertion in exon 4. C: Schematic of predicted Elovl2 protein in wild-type (260 AA) and elovl2^co100 (80 AA) fish. Genomic and protein results for the second generated mutant allele (elovl2^co101) are shown in Supplemental Fig. 1. All experiments were conducted using elovl2^co100 mutants unless otherwise noted.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Lipid Res.