Figure 2

Generation of stat3^S→A751 zebrafish knock-in line. A Sequence of the donor DNA used to generate the KI. The mutation (gca) is highlighted in red and is flanked by a 36-nt and a 90-nt homology arms. B Representative picture of allele-specific PCRs routinely performed to genotype KI fish: heterozygotes are positive for both alleles, whereas homozygotes are positive for only one. C Morphological analysis (in blue: eye diameter; in orange: eye area; in yellow: body length) and representative pictures of 6-dpf stat3^S751/S751, stat3^S751/A751 and stat3^A751/A751 larvae. Scale bar: 200 μm. D Standard light/dark behavioral assay performed on 6-dpf stat3^S751/S751, stat3^S751/A751 and stat3^A751/A751 larvae. E Genotype distribution of stat3^S751/S751, stat3^S751/A751 and stat3^A751/A751 zebrafish at 6 dpf, 15 dpf, and 60 dpf. F Fluorescent image of Tg(7xStat3-Hsv.Ul23:EGFP)^ia28 intestines of 6-dpf stat3^S751/S751, stat3^S751/A751 and stat3^A751/A751 larvae and relative fluorescence quantification. Scale bar: 100 μm. G Expression levels of stat3, socs3a, vegfa and cebpb in 6-dpf stat3^S751/S751 and stat3^A751/A751 larvae. H Regeneration rate of 6-dpf stat3^S751/S751, stat3^S751/A751 and stat3^A751/A751 larvae at 3 dpa. Mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.