Fig. 5

Instantaneous visual genotyping using the tyr allele-tracking reporter lines. (A-D) Visual genotyping results of the tyr mutation using ubi:EGFPtyr and ubi:mCherrytyr, two allele-tracking reporter lines. Heterozygous fish of these two lines were intercrossed, and their offspring were inspected under a fluorescent microscope. The same group of embryos was imaged in the bright field (A), green-fluorescent channel (B, shown in green), and red-fluorescent channel (C, shown in magenta). Images of B and C were merged in D. In panel A, embryos exhibiting the homozygous tyr mutant phenotype, as evidenced by the loss of pigmentation, were encircled by dashed lines. In panel D, embryos that were both green and magenta were white. Embryos that had no fluorescence in B to D were indicated by brown asterisks in A. Hence, the wild-type embryos were unmarked, the heterozygous embryos were either green or magenta, and the homozygous embryos were both green and magenta.