FIGURE

Fig. 5

ID

ZDB-FIG-240605-36

Publication

Wang et al., 2023 - Selenium deficiency-induced high concentration of ROS restricts hypertrophic growth of skeletal muscle in juvenile zebrafish by suppressing TORC1-mediated protein synthesis

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Fig. 5

Effects of 30 d of dietary treatments on protein synthesis in skeletal muscle of juvenile zebrafish. Muscle protein synthesis rate was detected 2 h after a meal using the surface sensing of translation method and quantified by measuring the incorporation of exogenous puromycin into nascent peptides. Puromycin-labelled peptides were detected by (a) Western blotting assay using an antibody against puromycin, and (b) the results are normalised to α-Tubulin. Values are means ± SEMs, n 3. *Significantly different (P < 0·05, two-way (dietary Se × dietary VE or MHY1485) ANOVA followed by Bonferroni–Dunn multiple comparison). ASe, the basal Se-adequate diet; DSe, the basal Se-deficient diet; VE, DL-α-tocopherol acetate, antioxidant. MHY1485, activator of the target of rapamycin.

Expression Data

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Br. J. Nutr.