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Fig. 5 Effects of 30 d of dietary treatments on protein synthesis in skeletal muscle of juvenile zebrafish. Muscle protein synthesis rate was detected 2 h after a meal using the surface sensing of translation method and quantified by measuring the incorporation of exogenous puromycin into nascent peptides. Puromycin-labelled peptides were detected by (a) Western blotting assay using an antibody against puromycin, and (b) the results are normalised to α-Tubulin. Values are means ± SEMs, n 3. *Significantly different (P < 0·05, two-way (dietary Se × dietary VE or MHY1485) ANOVA followed by Bonferroni–Dunn multiple comparison). ASe, the basal Se-adequate diet; DSe, the basal Se-deficient diet; VE, DL-α-tocopherol acetate, antioxidant. MHY1485, activator of the target of rapamycin.