FIGURE

Fig. 4

ID
ZDB-FIG-240221-4
Publication
Liu et al., 2024 - Live tracking of basal stem cells of the epidermis during growth, homeostasis and injury response in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Tracking of the basal cell behaviour in juvenile fish. (A) Tracking of the itgb4:cre-labelled cells at a single cell resolution in the 1-month-old juvenile fish fin. Fish were treated with 0.5 µM ICI to sparsely label the itgb4+ basal cells. dpl, days post labelling. The same region of the tissue was imaged in a 1-week time window (2-9 dpl). The EGFP+ cells displayed three proliferation patterns: quiescent (1 cell, blue arrowheads), slow-proliferating (2-4 cells, purple arrowheads) and fast-proliferating (>4 cells, orange arrowheads). White arrows indicate the posterior end (P) of fins. Experimental procedure is shown on the left. Scale bar: 200 µm. (B) Confocal images and their optical sections of fast-proliferating and slow-proliferating clones in the juvenile fish fin. Note that the fast-proliferating clone only contains non-basal cells, whereas the slow-proliferating clone only contains the basal cells. White arrows indicate the posterior side (P). Scale bars: 20 µm. (C) Ratios of clones with respective proliferation patterns (n=312 clones from five juvenile zebrafish). (D) Experimental scheme of long-term tracking of clonal cell fates. Time-lapse images were taken at intervals of 7 days. (E) Representative image of fast-proliferating clones. They quickly proliferated, but finally disappeared in the long run, suggesting that such clones did not contain the basal stem cells. Scale bar: 100 µm. (F) Representative image of quiescent clone. These clones kept quiescent for more than 79 dpl. Scale bar: 50 µm. (G) Representative image of slow-proliferating clone, which slowly expanded the clone size and maintained for more than 79 days. Scale bar: 30 µm. White arrowheads indicate examples of tracked clones.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development