PUBLICATION
Live tracking of basal stem cells of the epidermis during growth, homeostasis and injury response in zebrafish
- Authors
- Liu, Z., Meng, Y., Ishikura, A., Kawakami, A.
- ID
- ZDB-PUB-240125-2
- Date
- 2024
- Source
- Development (Cambridge, England) 151(2): (Journal)
- Registered Authors
- Kawakami, Atsushi
- Keywords
- Integrin beta 4, Keratin 18, Cell lineage, Cre-loxP, Zebrafish
- Datasets
- GEO:GSE241757
- MeSH Terms
-
- Animals
- Epidermal Cells
- Epidermis*
- Homeostasis
- Keratinocytes
- Zebrafish*
- PubMed
- 38265193 Full text @ Development
Citation
Liu, Z., Meng, Y., Ishikura, A., Kawakami, A. (2024) Live tracking of basal stem cells of the epidermis during growth, homeostasis and injury response in zebrafish. Development (Cambridge, England). 151(2):.
Abstract
Basal stem cells of the epidermis continuously differentiate into keratinocytes and replenish themselves via self-renewal to maintain skin homeostasis. Numerous studies have attempted to reveal how basal cells undergo differentiation or self-renewal; however, this has been hampered by a lack of robust basal cell markers and analytical platforms that allow single-cell tracking. Here, we report that zebrafish integrin beta 4 is a useful marker for basal cell labelling, irrespective of the body region, stage and regenerative status. We employed Cre-loxP recombination in combination with live cell tracking of single basal clones in the caudal fin and investigated the embryonic origin and behaviour of basal cells during fish growth and homeostasis. Although most basal cells, including those in fins, became quiescent in the adult stage, genetic cell ablation showed that basal cells were reactivated to either self-renew or differentiate, depending on the injured cell type. Our study provides a simple and easy-to-use platform for quantitative in vivo imaging of basal stem cells at wider stages and under various conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping