PUBLICATION

Live tracking of basal stem cells of the epidermis during growth, homeostasis and injury response in zebrafish

Authors
Liu, Z., Meng, Y., Ishikura, A., Kawakami, A.
ID
ZDB-PUB-240125-2
Date
2024
Source
Development (Cambridge, England)   151(2): (Journal)
Registered Authors
Kawakami, Atsushi
Keywords
Integrin beta 4, Keratin 18, Cell lineage, Cre-loxP, Zebrafish
Datasets
GEO:GSE241757
MeSH Terms
  • Homeostasis
  • Keratinocytes
  • Zebrafish*
  • Epidermis*
  • Epidermal Cells
  • Animals
(all 6)
PubMed
38265193 Full text @ Development
Abstract
Basal stem cells of the epidermis continuously differentiate into keratinocytes and replenish themselves via self-renewal to maintain skin homeostasis. Numerous studies have attempted to reveal how basal cells undergo differentiation or self-renewal; however, this has been hampered by a lack of robust basal cell markers and analytical platforms that allow single-cell tracking. Here, we report that zebrafish integrin beta 4 is a useful marker for basal cell labelling, irrespective of the body region, stage and regenerative status. We employed Cre-loxP recombination in combination with live cell tracking of single basal clones in the caudal fin and investigated the embryonic origin and behaviour of basal cells during fish growth and homeostasis. Although most basal cells, including those in fins, became quiescent in the adult stage, genetic cell ablation showed that basal cells were reactivated to either self-renew or differentiate, depending on the injured cell type. Our study provides a simple and easy-to-use platform for quantitative in vivo imaging of basal stem cells at wider stages and under various conditions.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tyt201TgTransgenic Insertion
    tyt214TgTransgenic Insertion
      tyt233TgTransgenic Insertion
        tyt234TgTransgenic Insertion
          tyt235TgTransgenic Insertion
            tyt236TgTransgenic Insertion
              tyt237TgTransgenic Insertion
                tyt238TgTransgenic Insertion
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  No data available
                  Fish
                  No data available
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  BFPEFGBFP
                  CreEFGCre
                  DsRed2EFGDsRed2
                  EGFPEFGEGFP
                  mCherryEFGmCherry
                  NTREFGNTR
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                  Mapping
                  No data available