Fig. 5

Loss of Atf6 limits temperature-induced acceleration of developmental rate and increases temperature sensitivity of the notochord (A) Images of Alcian blue-stained notochords (camera image in black and white) in the tails of wild-type and atf6 crispant embryos raised at 28°C and 35°C. Examples of notochord defects in crispants raised at elevated temperature, kinks indicated with red arrows (phenylthiourea was used for imaging 28°C crispants). (B) Box plots showing reduced capacity for acceleration of development at 34°C for the entire embryo (left) and notochord (right), while no developmental delay is apparent at 28°C. (C) Results of TEM of notochord sheath cells in atf6 crispants raised at elevated temperature, showing disrupted ER structure and aggregated collagen fibrils (red asterisks). Scale bar, 200 nm; sc, sheath cell; s, sheath.