FIGURE

Fig. 5

ID

ZDB-FIG-240126-13

Publication

Lau et al., 2023 - Proteomic analysis of zebrafish Folliculogenesis identifies YB-1 (Ybx1/ybx1) as a potential gatekeeping molecule controlling early ovarian Folliculogenesis

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Fig. 5

Western blot evidence for multi-site phosphorylation of Ybx1. The protein extract from PG follicles in the presence or absence of phosphatase inhibitors followed by 2D electrophoresis and Western blot analysis with YB1N, YB1C, and anti-p-YB-1. In the absence of phosphatase inhibitor, the signal of Ybx1 protein spanned a wide range of pH from 3 to 10. This pattern disappeared in the presence of phosphatase inhibitors and the signal became concentrated around pH 3. The anti-p-YB-1 detected phosphorylation at S82 site.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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