FIGURE

Fig. 4

ID

ZDB-FIG-240124-33

Publication

Rodríguez-Ruiz et al., 2023 - ZAKα/P38 kinase signaling pathway regulates hematopoiesis by activating the NLRP1 inflammasome

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Fig. 4

Lrrfip1 regulates hematopoiesis through the Nlrp1 inflammasome in zebrafish A–H Number of erythrocytes (A), neutrophils (B, F, H), macrophages (C), and HSPCs (D), and caspase‐1 activity (E) in nlrp1 crispant larvae of 2 dpf obtained by injecting one‐cell stage embryos with standard, nlrp1, lrrfip1a and/or lrrfip1b crRNAs/Cas9 complexes. Note that either lrrfip1a or lrrfip1b crRNAs/Cas9 complexes target both lrrfip1 paralogs (see Appendix Fig S10). (G, H) Larvae were also treated by bath immersion with 100 μM of the caspase‐1 inhibitor VX‐765. Representative images of neutrophils in lrrfip1 crispant larvae using Tg(lyz:dsRED) reporter line are shown in (G). Each dot represents one individual and the means ± SEM for each group are also shown. P values were calculated by Student's t‐test. n.s., non‐significant; *P<0.05; **P<0.01; ****P<0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.